Bacillus polymyxaの生産する高粘性多糖 : 微生物の生産する多糖に関する研究(第1報)

概要

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A bacterial strain producing a high-viscosity, agar-like polysaccharide was isolated from soil and identified as Bacillus polymyxa no. 458. Fifteen grams of the polysaccharide per liter were produced by the strain after 144 hr of shaking incubation in a mixture of glucose, Polypepton, beef extract and salts. For production of the polysaccharide, the optimum pH was 7.0-7.5 and the optimum temperature was 25-28℃.The polysaccharide was found to be acidic, and to be composed of glucose, mannose and glucuronic acid in an approximate molar ratio of 7 : 7 : 2. The viscosity of the polysacchardie solution at 0.5%, 1.0% and 1.5% was about 1,000cp, 11,000cp and 42,000cp respectively, which was 5-8 times higher than that of locast bean gum. Solutions of more than 0.7% formed an agar-like gel on heating at 90℃ for 10 min and cooling to 25℃.No acute or sub-acute toxicity was found on feeding the polysaccharide to mice.
社団法人日本生物工学会の論文
1981-04-25