酵母によるフェノールの分解

概要

論文の詳細を見る
The early oxidation steps of phenol by Trichosporon cutaneum WY 2-2 were examined.1)During the growth of WY 2-2 on a phenol medium, catechol, the hydroxylation product of phenol, was detected in the growth medium under restricted growth conditions.A high concentration (12 mg/l) of catechol accumulated in the medium at a restricted temperature (20℃) or under aeration with shaking for 24 hr followed by a stationary culture at 30℃.2)When the oxidation of catechol by a washed cell suspension of WY 2-2 grown on the phenol medium was followed by taking absorption spectra between 220 nm and 340 nm at intervals of 60 min for 300 min, an intermediate, which showed an absorption maximum at 260 nm in phosphate buffer at pH 7.5,accumulated to a maximal concentration after 60 min.The oxidation product of catechol was identified as cis, cis-muconic acid from the following data.(a)A thin-layer chromatogram of the reaction mixture after 60 min showed an unsaturated compound that co-chromatographed with authentic cis, cis-muconic acid in alkaline solvent system.(b)The ultraviolet absorption spectra of the oxidation product and authentic cis, cis-muconic acid in phosphate buffer at pH 7.5 were identical.At the same time the occurrence of succinic acid was indicated in the reaction mixture after 300 min by thin-layer chromatography.3)It was suggested from the results of the ultraviolet absorption of the reaction mixtures with extracts of WY 2-2 that catechol, 4-methylcatechol, 3-methylcatechol and pyrogallol were converted cis, cis-muconic acid, 3-methylmuconic acid, 2-methylmuconic acid and 2-hydroxymuconic acid, respectively.Properties of enzymes involved in the aromatic-ring fission from several Trichosporon and Candida strains were investigated.1) All strains employed possessed an intradiol dioxygenase, catechol 1,2-dioxygenase.2) These strains could be classified into two groups with a defference in the substrate specificity;one group exhibited higher activities towards 4-methylcatechol than towards catechol and the other higher activities towards catechol than towards 4-methylcatechol.
社団法人日本生物工学会の論文
1979-11-25

酵母によるフェノールの分解

スポンサーリンク

概要

著者

関連論文

スポンサーリンク