メタン資化性細菌の分離とその性質 : メタン資化性細菌の混合培養に関する研究(第1報)

概要

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Active enrichment cultures were obtained using soil as seed. Culture No. 12 growing most abundantly on methane was selected for further study. The culture, however, contained two kinds of bacteria as seen by microscopic observation. They were separated by Lindner's drop culture method. One bacterium, strain M, which was determined to be Methylosinas sp., was able to grow on methane and methanol as a sole carbon source, but the other, strain G, which was identified as Pseudomonas sp., could grow on glucose, methanol, and formaldehyde, but not on methane. The growing ability of strain M decreased within 1 month when it existed alone. Strain M showed poor growth on methane when it grew alone, but its growth on methane was enhanced 6-7 times as much as its pure culture when it was mixed with strain G. Strain M could also grow on methane abundantly in the medium containing the supernatant fluid of the cultured broth of strain G with methanol. Strain M was supposed to supply methanol, which is the intermediate metabolite of methane, to strain G.Culture No. 12 is thought to be a new system of mixed culture different from others reported so far, based on differences of cultural conditions, cell composition and amino acid composition of cell protein.
公益社団法人日本生物工学会の論文