エノキタケ鋸屑培地培養物抽出液のタバコモザイクウイルス感染阻止作用

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An investigation was made on the inhibitory effect on tobacco mosaic virus (TMV) infection exerted by the aqueous extract from the cultre of Flammulina velutipes grown in the saw dust-rice bran medium. The extract was noted to have an inhibitory activity in fractions of a wide range eluted from the Sephadex G-75 column. The molecular weight of an active substance in the extract was presumed to be 15000-50000 from the elution pattern on the Sephadex column chromatogram. The fractions with an inhibitory activity showed positive color reaction to protein and sugar. Such activity remained unchanged even after the procedure to remove protein or protease treatment. This suggests that the activity of the substance depends primarily on sugar. Detected as a result of thin-layer chromatography of hydrolyzate of the above fractions were galactose, glucose, mannose, xylose and an unidentified sugar. The inhibitory activity of the extract varied somewhat according to test plants. TMV infection was inhibited not only by the application of the mixture of the extract and TMV to plant leaves but also by its application prior to virus inoculation. In case the extract was applied after virus inoculation, however, it failed to exhibit satisfactory effect. Its inhibitory effect by the pre-treatment was diminished when the treated leaves were washed before virus inoculation. Dilution of the mixture of the extract and TMV with water resulted in restoration of virus infectivity. When the above mixture was eluted through the Sephadex G-75 column, Virus infectivity was recognized in fractions containing TMV.
1978-07-25

著者

高木康至
大塚化学薬品

エノキタケ鋸屑培地培養物抽出液のタバコモザイクウイルス感染阻止作用

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