Continuous Enzymatic Production of Invert Sugar

概要

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Continuous inversion of sucrose by invertase immobilized on collagen membranes in semi-pilot scale reactors was investigated using industrial feed streams. Several commercially available invertase preparations were evaluated. The highest activity obtained was 2,400 IU per g of collagen-invertase membrane. In continuous reactor (capacity 1.2l) operation performed at a temperature of 55℃ using 1.52 M sucrose solution as substrate, the operational half-life of the catalyst was estimated to be 1,870 hr. At lower substrate feed concentrations, the reactor operational stability was even better. The color of the process stream remained unchanged during the inversion process. Product quality compared favorably to acid hydrolyzed invert sugar. At high reactor residence times, greater than 90% sucrose conversion could be achieved without any objectionable color formation.Kinetic data from both batch and continuous reactors indicate the presence of mass transfer resistances which disguise the overall reaction kinetics. The sucrose concentration at which substrate inhibition is observed ranged from 0.285 M in the case of the soluble enzyme to 0.564 M in the case of the immobilized enzyme. At low substrate concentrations, sucrose hydrolysis could be described by Michaelis Menten kinetics. The significance of the above results are discussed with respect to reactor scale-up.
1977-10-25