Biokinetic Parameter Estimation for Degradation of 2,4,6-Trinitrotoluene (TNT) with Pseudomonas putida KP-T201(ENVIRONMENTAL BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
The purpose of this study is to determine the biodegradation kinetics of 2,4,6-trinitrotoluene (TNT) by a newly isolated microorganism. Hundreds of microorganisms were isolated from explosives-contaminated soil in Korea. Examination of culture tests revealed that a few species of microorganisms have good ability to degrade TNT. The most efficient one was selected and identified as Pseudomonas putida KP-T201. Biodegradation of TNT was tested in a batch reactor using a pure culture of P. putida KP-T201. The effect of different concentrations of TNT on the rate of bacterial biodegradation was investigated. The Haldane equation seems to be an adequate expression for the cell growth data, and the kinetic constants obtained were μ_m=0.65 h^<-1>, K_S=0.62 mgl^<-1>, and K_i=115 mgl^<-1>. The dependence of the bacterial specific growth rate on the concentration of TNT could be explained as a conventional model of substrate inhibition.
- 社団法人日本生物工学会の論文
- 2002-07-25
著者
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LEE Jinwon
Department of Electrical Engineering and Computer Science, KAIST
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Kim T‐h
Advanced Energy & Environment Research Team Korea Institute Of Industrial Technology
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Lee J
Department Of Chemical Engineering Kwangwoon University
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Lee Jinwon
National Research Laboratory Of Plant Functional Genomics Division Of Molecular And Life Sciences Po
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Lee Jinwon
Department Of Electrical Engineering And Computer Science Kaist
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Kim S‐w
Advanced Energy & Environment Research Team Korea Institute Of Industrial Technology
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Park Chulhwan
Advanced Energy & Environment Research Team Korea Institute Of Industrial Technology:department
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KIM SEUNG-WOOK
Department of Chemical and Biological Engineering, Korea University
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KIM TAK-HYUN
Advanced Energy and Environment Research Team, Korea Institute of Industrial Technology
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KIM SANGYONG
Advanced Energy and Environment Research Team, Korea Institute of Industrial Technology
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Kim Seung-wook
Department Of Chemical And Biological Engineering Korea University
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Kim Tak-Hyun
Advanced Energy & Environment Research Team, Korea Institute of Industrial Technology
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Kim Sangyong
Advanced Energy & Environment Research Team, Korea Institute of Industrial Technology
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