Potasisum Ion Enhances Tissue-Type Plasminogen Activator Expression in Cultured HEL Cells

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The effects of inorganic cations on the production of t-PA in cultured human embryonic lung (HEL) cells were studied. Adding K^+ or Ca^<2+> to the cultured medium for HEL cells increased t-Pa relase into the medium in a dose-dependent manner. To investigate the intracellular response caused by increment of extracellular K^+ concentration, intracellular Ca^<2+> concentration and intracellular pH were measured. Increasing extracellular K^+ caused an increase in intracellular pH, but did not affect intracellular Ca^<2+> levels. It was also found that extracellular Ca^&la;2+> increased the level of t-PA mRNA by northern blot analysis using human t-PA cDNA fragment as a proble, while extracellular K^+ did not. When the extracellular pH was decreased from 7.4 to 6.6,intracellular pH detected using the BCECF method was found to have shifted to a higher pH than the extracellular pH. Furthermore, the maximum production of t-PA was obtained at a pH range in which the difference between intracellular pH and extracellular pH was the greatest. Maximum t-Pa relase was observed at a K^+ of about 32.2 mM in dishes. A similar effect of K^+ on t-PA relase was also observed in 10 l spinner flasks. Furthermore, the addition of K^+ to the cultured medium markedly increased the specific glucose consumption rate. It is concluded that K^+ seems to affect t-PA production at the post-transcriptional level through changes in intracellular pH and glucose consumption.
公益社団法人日本生物工学会の論文
1993-08-25

Potasisum Ion Enhances Tissue-Type Plasminogen Activator Expression in Cultured HEL Cells

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