Improvement of Astaxanthin Production by Phaffia rhodozyma through Mutation and Optimization of Culture Conditions

概要

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Phaffia rhodozyma strains were treated with the mutagenic agent NTG several times and plated onto yeastmalt agar containing β-ionone as a selective medium. One of the NTG-treated strains (NCHU-FS301) produced considerably more astaxanthin than the parent CBS-6938 (strai NCHU-FS301 produced 1515.63 μg/g and CBS-6938 565.08 μg/l). When the kinetic parameters of the specific growth rate (μ) and specific astaxanthin productivity (q_p) were used to judge the association between growth behavior and product formation, NCHU-FS301 was shown to be a more positive growth-associated fermentation type than the parent strain. A study of the effects of the carbon source on red pigment formation revealed that glucose could support the highest total astaxanthin production (7809.3 μg/l). Yeast extract was the best nitrogen source in supporting the highest total astaxanthin formation (8637.5 μg/l). When mixed nitrogen sources were used, a mixture of yeast extract, beef extract, and potassium nitrate (1 : 1 : 1) supported more pigmentation (8052.6 μg/l) than the other mixtures tested. Astaxanthin-overproducing mutants could be useful in providing a natural source of astaxanthin for the aquacultural industry.
公益社団法人日本生物工学会の論文
1993-06-25