Continuous ATP Production by Spinach Thylakoid in a Stirred Tank Reactor
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概要
- 論文の詳細を見る
Spinach thylakoid was immobilized by two different methods for the purpose of retantion within a continuous-flow stirred tank reactor (CSTR). The glutaraldehyde crosslinked albumin polymer method completely inactivated the cyclic ATP photophosphorylation of thylakoid. In contrast, agarose-entrapped thylakoid retained about 17% of the activity of the cyclic photophosphorylation of non-immobilized thylakoid. This activity devlined continuously during ATP production in the CSTR. Fifty percent of the initial activity was lost within about 5.5 h. Ascorbate was found to increase the stability of ATP photophosphorylation; about twice as much ATP was produced at the optimal ascorbate concentration of 5 mM. Under the optimal dilution rate of 2.36 h^<-1>, about 60 μmol of ATP per mg chlorophyll were produced in 20 h by agarose-entrapped thylakoid in the CSTR. These results showed that, compared to non-immobilized thylakoid in batch operation, agarose-entrapped thylakoid produced only a low amount of ATP under continuous operation.
- 公益社団法人日本生物工学会の論文
- 1992-06-25
著者
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Hosono Kuniaki
Fermentation Research Institute Agency Of Industrial Science And Technology
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YU ALEX
(Present address)Pulp and Paper Research Institute of Canada
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