Ammonium Controlled Fed-Batch Fermentation for Amylase Production

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概要

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• Batch and ammonium controlled fed-batch fermentations were compared for the production of amylase by Bacillus amyloliquefaciens. In shake flask culture, maximum amylase production was obtained when the concentration of ammonium was 20-30 mM. In a 15 l batch fermentation, maximum amylase production occurred at an ammonium concentration of 40 mM. In fed-batch fermentations, the optimum concentration of ammonium for the growth of B. amyloliquefaciens was 10-20 mM. The maximum production of amylase and biomass was found when the ammonium concentration was controlled at 5 mM. Amylase production at 5 mM ammonium was 5000 IU/l, which represents a two-fold increase in enzyme production over the uncontrolled experiments.

• 公益社団法人日本生物工学会の論文
• 1989-12-25

著者

• Gerson Donald

  Connaught Laboratories Limited

• Kole Manoj

  Connaught Laboratories Limited

関連論文

• Ammonium Controlled Fed-Batch Fermentation for Amylase Production

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