Determination of a Small Quantity of Cystine in the Presence of a Large Amount of Cysteine
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概要
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A procedure is described to precisely determine a very small amount of cystine in the presence of a large amount of cysteine. After completely modifying cysteine with N-ethylmaleimide, the remaining reagent was reacted with DL-homocysteine. Cystine was determined, after being reduced with dithiothreitol, by the reaction with ninhydrin carried out under acidic conditions. The procedure makes it possible to precisely determine the amount of cystine present with cysteine in a concentration ratio of 1 : 2,000. By employing this procedure, auto-oxidation of cysteine to cystine in a mixture for the L-cysteine α, β-elimination reaction was investigated.
- 社団法人日本農芸化学会の論文
- 1999-08-23
著者
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Yamagata Shuzo
Department Of Biotechnology Faculty Of Agriculture Gifu University
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Iwama Tomonori
Department Of Biotechnology Faculty Of Agriculture Gifu University
関連論文
- Substrate Inhibition of L-Cysteine α,β-Elimination Reaction Catalyzed by L-Cystathionine γ-Lyase of Saccharomyces cerevisiae(Biochemistry & Molecular Biology)
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- Conversion of the Aminocrotonate Intermediate Limits the Rate of γ-Elimination Reaction Catalyzed by L-Cystathionine γ-lyase of the Yeast Saccharomyces cerevisiae
- Determination of a Small Quantity of Cystine in the Presence of a Large Amount of Cysteine
- A MODIFICATION OF AN AMINO TERMINAL REGION OF THE SACCHAROMYCESCEREVISIAE MET17/MEET25 GENEPRODUCT
- Conversion of the Aminocrotonate Intermediate Limits the Rate of γ-Elimination Reaction Catalyzed by L-Cystathionine γ-lyase of the Yeast Saccharomyces cerevisiae