Production and Properties of the Linamarase and Amygdalase Activities of Penicillium aurantiogriseum P35

概要

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The effects of medium composition on the production of β-glucosidase (amygdalase and linamarase) by Penicillium aurantiogriseum P35 were studied and the medium optimized as follows (g/l of deionized water) : pectin, 10.0 ; (NH_4)_2SO_4,8.0 ; KH_2PO_4,8.0 ; Na_2HPO_4,2.8 ; MgSO_4・7H_2O, 0.5 ; yeast extract, 4.0 ; initial pH 6.0. When grown in a bench fermenter on this medium, the fungus produced 50.5 mU of amygdalase and 9.4 mU of linamarase per ml of culture broth. Two β-glucosidases (PGI and PGII), each having amygdalase and linamarase activities, were recovered from the culture broth and purified ; their relative molecular weights, as native enzymes, were estimated to be about 247000 and 147000,respectively. Both enzymes showed the same optimum pH (6.0) but different optimum temperatures (55 and 60℃ for PGI and PGII, respectively). Thermostability (10 min at 60℃) and half-life of enzyme activity (7 hours at 60℃) of PGII were higher than those of PGI (10 min at 50℃ and 2 hours at 55℃, respectively). A wide range of cyanogenic glycosides (such as tetraphyllin B, epivolkenin, gynocardin, passibiflorin, prunasin, taxiphyllin, amygdalin, lucumin, sambunigrin, dhurrin, linamarin and cardiospermin sulfate) were hydrolyzed by both enzymes.
社団法人日本農芸化学会の論文
1999-05-23