Flavonoids as Inhibitors of NADP-Malic Enzyme and PEP Carboxylase from C_4 Plants
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概要
- 論文の詳細を見る
The inhibitory effects of flavonoids on the activity of two photosynthetic enzymes such as phosphoenolpyruvate carboxylase (PEPCase) and NADP-dependent malic enzyme (NADP-ME) were evaluated. The glycosylation of hydroxyl groups on the flavonoids resulted in compounds that behaved as gradually weaker inhibitors with increased size of the substituent. Quercetin and baicalein showed a competitive inhibition pattern vs. NADP^+ for NADP-ME, and a similar model for both tlavonoids vs. phosphoenolpyruvate (PEP) was observed when tested on PEPCase. K_i for NADP-ME inhibition at pH 7.0 were 0.83 μM and 1.54 μM for quercetin and baicalein, respectively. K_i for PEPCase inhibition were 0.17 μM and 0.79 μM (quercetin and baicalein, respectively), indicating that these compounds are the most potent inhibitors described for this carboxylase. I_<50> values for these and other flavonoids were in the micromolar range. A tentative physiological role for the inhibitory effects observed on PEPCase is discussed.
- 社団法人日本農芸化学会の論文
- 1996-05-23
著者
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Andreo C
Univ. Nacional De Rosario Rosario Arg
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Colombo S
Centro De Estudios Fotosinteticos Y Bioquimicos (cefobi Conicet-fund. M. Lillo) Facultad De Ciencias
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Andreo Carlos
Centro De Estudios Fotosinteticos Y Bioquimicos Facultad De Ciencias Bioquimicas Y Farmaceuticas
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Pairoba C
Centro De Estudios Fotosinteticos Y Bioquimicos (cefobi Conicet-fund. M. Lillo) Facultad De Ciencias
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PAIROSA Claudio
Centro de Estudios Fotosinteticos y Bioquimicos (CEFOBI, CONICET-Fund. M. Lillo), Facultad de Cienci
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COLOMSO Sergio
Centro de Estudios Fotosinteticos y Bioquimicos (CEFOBI, CONICET-Fund. M. Lillo), Facultad de Cienci
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Andreo Carlos
Centro De Estudios Fotosinteticos Y Bioquimicos (cefobi) Universidad Nacional De Rosario
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