Isolation and Characterization of Mutants Partially Deficient in Aldehyde Dehydrogenase in Saccharomyces cerevisiae

概要

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Mutants of the yeast Saccharomyces cerevisiae that are partially deficient in aldehyde dehydrogenases (ALDHs) were isolated. A mutant, AK-9, was shown to be especially defective in the NADP^+-linked ALDH. The K^+-activated ALDH of a mutant AK-25 was found in the cytosol, but not in the mitochondria. The strain AK-25 continuously accumulated acetate even in the phase of ethanol consumption in batch aerobic cultures. A mutant, AK-26, was defective in two isozymes of ALDH, particularly in the K^+ -activated ALDH. The aldh mutants of AK-9 and AK-25 were more sensitive to the growth inhibitory effects of ethanol and NaCl, but the mutant AK-26 was only sensitive to ethanol. All aldh mutants stimulated more acetaldehyde production than the wild-type strain during conditioning in media containing ethanol. The mutants except for AK-26 almost never produced acetaldehyde during conditioning in media containing 2+6% NaCl. The mutant AK-26 produced glycerol almost the same as the wild-type strain during conditioning in NaCl. These results suggested that two isozymes of ALDH were related to ethanol tolerance of which the key substance was acetaldehyde induced by ethanol stress, and that glycerol production induced by salt stress was closely related to K^+ -activated ALDH.
社団法人日本農芸化学会の論文
1994-04-23