Isozymes of Bighead Shrimp Alkaline Phosphatase

スポンサーリンク

概要

• 論文の詳細を見る

• Three alkaline phosphatase isozymes, named APase-I, II, and III, were isolated from the cephalothoraxes of bighead shrimp (Solenocera melantho). The molecular weights of APase-I, II, and III estimated by SDS-PAGE were 88.6, 53, and 20kDa, respectively. They were all glycosylated monomeric enzymes. The optimum pH of APase-I and II were 10 and 8, and the pH ranges for maximum stability were 8.0-12.5 and 5.0-8.0, respectively. Both enzymes showed optimum temperature around 37℃. Arrehnius plots for enzyme thermal inactivation showed that APase II was more stable than APase I above 18℃ but less stable below this temperature. Both enzymes showed the highest activities toward aryl phosphate esters among several phosphoesters tested. The K_m for p-nitrophenyl phosphate were 0.14 and 4.44mM for APase I and II, respectively. Na^+ stimulated both enzyme activities in physiological concentration range. APase I was less susceptible to denaturating agents and inhibitors than Apase II.

• 社団法人日本農芸化学会の論文
• 1994-01-23

著者

• Shaw J‐f

  Institute Of Botany Academia Sinica Nankang Taipei Taiwan R.o.c And Institute Of Marine Biotechnolog

• Shaw Jei-fu

  Institute Of Botany Academia Sinica:department Of Marine Food Science National Taiwan Ocean Universi

• Chen Wen-Chieh

  Department of Marine Food Science, National Taiwan Ocean University

• Chen Wen-chieh

  Department Of Marine Food Science National Taiwan Ocean University

関連論文

• Characterization of Fish Cu/Zn-Superoxide Dismutase and Its Protection from Oxidative Stress
• Production of High-maltose Syrup and High-protein Flour from Rice by an Enzymatic Method
• Isozymes of Bighead Shrimp Alkaline Phosphatase

スポンサーリンク