Synthesis and Sedimentation of a Subset of 15-kDa Heat Shock Proteins in Escherichia coli Cells Recovering from Sublethal Heat Stress

概要

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After heat treatment at 55℃ for 15s, Escherichia coli cells were incubated at 37℃ in a chemically defined medium for cell recovery from heat injury. During this period, four heat-inducible proteins with similar molecular masses of 15kDa or so but different isoelectric points were found to appear and to be sedimented by ultracentrifugation. This subset of proteins were σ^<32>-dependent and two of them were C14.7 and G13.5 proteins, which have been known as heat shock proteins (HSPs), but the other two, which were designated here as B and F proteins, are unknown. The 15-kDa proteins were solubilized by the treatment of the sediment with 6M urea. The result obtained by peptide mapping of these proteins suggests that B and F proteins may be modified forms of C14.7 and/or G13.5 proteins and that these proteins have a homologous structure.
社団法人日本農芸化学会の論文
1993-04-23