Kinetic Analysis of the Growth of Spirulina sp. in Batch Culture

概要

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Batch cultivation of Spirulina sp. was carried out under limited light at 30℃ in the pH range of 9.2 to 9.7. The specific growth rate D was calculated from the tangent of the growth curve and the cell concentration at that time, and the amount of light energy absorbed per unit time per unit cell weight (E_x), namely, the specific absorption rate of light energy, was also calculated from the total amount of radiant flux of transmitted light at the surface of the culture vessel and cell concentration of the culture solution. A plot against E_x of D in the linear growth phase in batch culture and at various phases in continuous culture gave, for E_x of less than 1.0 kcal/g・h, points scatted near a straight line with slope m 0.037 g/kcal and an intercept on the ordinate, -b, of -0.0046 h^<-1>, and, for higher E_x values, points scattered near a curve of gradually decreasing slope which tended to approach a constant value.A Lineweaver-Burk plot of the reciprocal of D plus b against that of E_x yielded an equation for the growth rate which represented well the growth curve in batch culture. This equation also expressed the linear increase of D with increase of E_x at high cell concentration in the culture solution. The relation between cell growth rate and cell fluidity is discussed by use of a vector equation obtained by applying this relation to a culture solution contained in a given closed surface.
1987-06-25