Kinetics of L-Aspartic Acid β-Decarboxylation with Immobilized Pseudomonas dacunhae Cells
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For L-aspartic acid β-decarboxylation with immobilized Pseudomonas dacunhae cells, (1) the kinetic constants at optimal pH (6.2) and 37℃, Michaelis constant (K_m') and maximum reaction rate (V_m'), were 4.9×10^<-6> mol/cm^3 and 1.37×10^<-3> mol/cm^3・h, respectively. (2) The reaction rate was influenced by pH, and the relationship between the reaction rate and temperature was correlated by the Arrhenius equation at less than 48℃. (3) The influence of the particle size of the immobilized cells on the reaction rate could be explained by a theory of catalytic effectiveness factor. The simplified empirical rate equations for L-aspartic acid β-decarboxylation have been derived from the experiments on kinetic constants.
- 公益社団法人日本生物工学会の論文
- 1985-10-25
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