Renin-Angiotensin-Aldosterone系に関する研究 : 慢性期Goldblatt's Ratsにおける腎Reninと副腎Aldosterone産生の動態に関する2,3の検討

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The mechanism of the elevation of the blood pressure in hypertension caused by renal artery constriction in animals is generally thought to occur by release of renin in acute stage, but on the results in chronic stages one doesn't come always to an agreement. This study was undertaken to show a possible role of renin-angiotensin-aldosterone sys-tem in chronic stages of the experimental renal hypertension. For this purpose, the following experiments were examined, using Goldblatt's rats in each period of chronic stages, that is, 3-4th month, 5-6th month and 18th month : determinations of renin content of the kidneys and production rate of aldosterone in adrenals, in relation to Juxtaglomerular index (J. G. index) of kidneys and histological findings of kidneys and adrenal cortex. Materials and Methods. Adult male rats of the Wister strain weighing about 150 g were applied with clips to each left renal artery in order to produce persistent hypertension. The rats were maintained on a standard chow (M.F. ) of "Oriental Enzyme Co." (in Japan) in our laboratory during 3 to 18 months. The blood pressure was determined by the tail plethysmography. Renin-extraction from the kidney was performed according to HAAS & GOLDBLATT'S method (1954) (Fig.1) and LEVER'S mothod (1964) was employed in preparing renin substrate from dog's plasma (Fig. 2). Renin extracts were bioassayed by rats after the incubation with the substrate by means of SCHAFFENBURG'S method (1960) (Fig.3). Aldosterone production rate of adrenals was determined by our double isotope derivative dilution method (ISHIBASHI'S method, 1967) (Fig. 4). Histology of the kidneys and adrenals was observed by the hematoxylin-eosin staining, and also adrenal cortex was observed by electron-microscope. Results The mean blood pressure of our experimental rats ranged from 150 to 180 mmHg after constriction. Renin content of the rat kidneys in 3 to 4th month after clamping increased and that of the contralateral kidneys decreased, when compared with that of controlled rats. There was a similar tendency in the rats in 5 to 6th month and 18th month (Fig. 5, Fig. 6). J. G. index of clamped kidneys increased and that of contralateral kidneys decreased in each of all chronic stages. Aldosterone production rate of adrenals increased, when compared with that of controlled rats in each of all chronic stages (Fig. 5, Fig. 6). In the histological observations, the kidneys showed sclerotic changes, especially in the clamped kidneys on 18th month of Goldblatt's rats (Fig. 7, Fig. 8, Fig. 9, Fig. 10). In adrenal cortex, hypertrophy of zona glomerulosa was demonstrated, especially in 18th month (Fig. 11, Fig. 12).
社団法人日本循環器学会の論文
1967-12-15

Renin-Angiotensin-Aldosterone系に関する研究 : 慢性期Goldblatt's Ratsにおける腎Reninと副腎Aldosterone産生の動態に関する2,3の検討

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