INCREASED LEVELS OF INHIBITORY G PROTEIN IN MYOCARDIUM WITH HEART FAILURE

元データ 1994-11-20 社団法人日本循環器学会

概要

To identify any differences in inhibitory G protein (Gi) attributable to species or the cause of heart failure, we studied the changes in this protein in different animal models of heart failure: 1) different species; rats vs. hamsters (F1B) with cardiomyopathy induced by adriamycin (ADR) and 2) different etiologies; rats with ischemic heart failure (IHD) due to coronary artery ligation vs. rats with cardiomyopathy induced by ADR and F1B (20-week-old) hamsters with cardiomyopathy induced by ADR vs Syrian hamsters BIO 14.6 (40-week-old) with genetic cardiomyopathy, using Western blotting methods and ADP-ribosylation. We also sought to determine whether changes in the amount of Gi protein reflected the regulation of adenylate cyclase. The amount of immunodetectable Gi rose by 35% (p<0.05) in ADR rats, 25% (p<0.05) in ADR hamsters, 15% (p<0.05) in IHD rats, and 28% (p<0.05) in BIO 14.6 hamsters, as compared with control rats, F1B (20-week-old) hamsters, sham-operated control rats, and F1B (40-week-old) hamsters, respectively. Assessment of Gi by pertussis toxincatalyzed ADP-ribosylation revealed increases in Gi of 24% (p<0.05) in ADR rats and of 44% (p<0.05) in BIO 14.6 hamsters, as compared with their respective controls. Gi function, as assayed by the acetylcholine-induced inhibition of adenylate cyclase, also increased. Thus, Gi protein appears to contribute to the changes in signal transduction in myocardium with heart failure.

著者

Iwasaki Tadaaki First Department of Internal Medicine, Hyogo College of Medicine
Nakamura Kiyoko First Department of Internal Medicine Hyogo college of medicin
Iwasaki T Department Of Internal Medicine Division Of Coronary Heart Disease Hyogo College Of Medicine
Iwasaki T 兵庫医科大学第一内科
Iwasaki Tadaaki Department Of Internal Medicine Division Of Coronary Heart Disease Hyogo College Of Medicine
Kawamoto Hideo First Department Of Internal Medicine Hyogo College Of Medicine
OHYANAGI Mitsumasa First Department of Internal Medicine, Hyogo College of Medicine
Iwasaki Tadaaki First Department Of Internal Medicine And Department Of Nuclear Medicine Hyogo College Of Medicine
Yamamoto Juro First Department Of Internal Medicine Hyogo College Of Medicine
Kawamoto Hideo First Department Of Internal Medicin Hyogo College Of Medicine
Nakamura Kiyoko First Department Of Internal Medicine Hyogo College Of Medicine
Kawamoto Hideo 1st Department Od Jnternal Medicine Hyogo College Of Medicine
Ohyanagi Mitsumasa First Department Of Internal Medicine And Department Of Nuclear Medicine Hyogo College Of Medicine

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