Characterization of Anti-Myocardial Autoantibodies in Japanese Patients With Dilated Cardiomyopathy

概要

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Few previous reports have comprehensively screened all the anti-myocardial autoantibodies(AMCA)in relation to other clinical profiles in patients with idiopathic dilated cardiomyopathy(IDC), so the present study used both immunohistochemistry(FITC)and immunoblotting(IB)for screening patients with IDC in order to characterize the clinical significance of AMCA. Sera were collected from 100 patients with IDC and age-matched 100 healthy control subjects(CTL). For FITC, an unfixed frozen section of human myocardium was used for the standard indirect immunofluorescence; for IB, total cardiac homogenates of the same myocardium were blotted to serum at 2 sets of dilution(1:200 and 1:10,000). The positive rates of AMCA detection for each method were as follows(IDC vs CTL); 39% vs 6% for FITC, 38% vs 4% for IB(1:200), and 10% vs 0% for IB(1:10,000). Fifty-nine patients with IDC and 8 CTL were positive for AMCA by either method, and 18 patients with IDC and 2 CTL were positive for AMCA by both methods. IB-positivity at 1:200 was an independent predictor by multiple logistic regression analysis of non-sustained ventricular tachycardias well as left ventricular enddiastolic diameter and plasma norepinephrine concentraion.
社団法人日本循環器学会の論文
2001-09-20