平板上のコロニー密度の上限について

概要

論文の詳細を見る
The upper limit of viable cell count by plate counting method was studied theoretically and experimentally. The probability which k-cfus make n-colonies, A(k, n), was expressed by the following equation : A(k, n)= n・p・A(k-1,n)+(1+(n-1)・p)・A(k-1,n-1) where p is the ratio of colonies' average area to plate area. From this equation it was indicated that as the values of p increased, fused colonies increased and the counts of colonies decreased. Similarly this phenomenon was also observed at the experiment and calculated values coincided with the experimental values qualitatilvely. At this equation, using p/5 instead of p calcutated values coincided with the experimental values quantitatively. Therefore, it was concluded that this equation refrects the acutual plate counting and the upper limit of it had a theoretical base. In the present report this equation gave the upper limit of plate counting method a theoretical base.
日本微生物生態学会の論文
1988-08-01