TED-AJ03-208 Response of Murine Osteoblasts to Cryopreservation : The Influence of Attachment to Hydroxyapatite (HA) Scaffolds
スポンサーリンク
概要
- 論文の詳細を見る
This paper presents data relevant to the rational design of cryopreservation processes for tissue-engineered bone. The effects of cell-scaffold interactions and cell-cell interactions on osteoblast viability and attachment to hydroxyapatite (HA) scaffolds following cryopreservation processing are defined experimentally. It is found that cryopreservation processing detaches osteoblasts from the HA scaffold and that cells suffer significantly more damage than when they are cryopreserved in liquid suspension. Thus, some aspect of cell-surface interaction is detrimental. The detrimental influence involves thermal modulations because when osteoblasts attached to HA are exposed to cryoprotective agents for two-step freezing or vitrification without temperature change, morphological changes occur but little detachment or damage is observed. Enhanced post-thaw attachment and viability are realized for higher number densities of osteoblasts on the HA scaffold surface, indicating that some aspect of cell-cell interaction is beneficial. Higher porosity HA scaffolds are better for cell attachment but porosity is not a statistically significant factor for post thaw viability. These results establish the significance of cell-surface and cell-cell effects on determining post thaw attachment and viability of osteoblasts on HA scaffolds. This forms the basis for future studies aimed at elucidating the underlying mechanisms.
- 一般社団法人日本機械学会の論文
著者
-
Liu B.
Department Of Materials Science And Engineering Tsinghua University
-
Mcgrath J.
Department Of Mechanical Engineering Michigan State University:presently At The Aerospace And Mechan
-
Baumann M.
Department Of Chemical Engineering And Materials Science Michigan State University
-
Mcgrath J.
Department Of Cell And Molecular Pathology St John's Institute Of Dermatology The Guy's Ki
-
McCabe L.
Department of Physiology, Michigan State University
-
Mccabe L.
Department Of Physiology Michigan State University
関連論文
- Nanotribological characteristics of materials measured with force microscopy (マルチメディアストレージ 第6回アジア情報記録技術シンポジウム〔英文〕)
- Nanotribological Characteristics of Materials Measured with Force Microscopy
- The Effects of Duration and Intensity of Stimulation on Total Protein and Mucin Concentrations in Resting and Stimulated Whole Saliva
- Immunoquantification of Human Salivary Mucins MG1 and MG2 in Stimulated Whole Saliva: Factors Influencing Mucin Levels
- Bradykinin Mediates Phosphorylation of eNOS in Odontoblasts
- NO-cGMP Signaling Molecules in Cells of the Rat Molar Dentin-Pulp Complex
- Heterozygous Mutation in the SAM Domain of p63 Underlies Rapp-Hodgkin Ectodermal Dysplasia
- TED-AJ03-208 Response of Murine Osteoblasts to Cryopreservation : The Influence of Attachment to Hydroxyapatite (HA) Scaffolds
- Preparation and Separation Performance of a TPAOH-Induced ANA Zeolite Membrane
- Atomistic Modeling of Metastable Phase Selection of a Highly Immiscible Ag-W System(Cross-disciplinary Physics and Related Areas of Science and Technology)
- Structural Stability of the Metastable Solid Solution in the Equilibrium Immiscible Ag-Mo System Predicted by an ab Initio Derived Potential (Condensed Matter : Structure, Mechanical and Thermal Properties)