インスリン抗体に関する研究(第2報)
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概要
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The antigenicity of insulin in guinea-pig was studied. A group of ginea-pigs was immunized with insulin conpled sheep erythrocytes by bis-diazotized benzidine and an other group was done with insulin in Freund's adjuvant. The antibodies were demonstrated by BDB-hemagglutination test, Schultz-Dale's reaction, agar gel diffusion and two tests for insulin neutalization, in which the neutralization of exogenous ox insulin was demonstrated by mouse convulsion test and the neutralization of endogenous insulin in mouse was measured by hyperglycemia after injection of the antiserum. Postive Schultz-Dale's reactions and negative agar gel diffusion tests were resulted in both antisera. Antiserum immunized with insulin in Freund's adjuvant had low BDB-hemagglutination titer, positive mouse convulsions test and positive neutralizing activity of endogenous insulin in mouse. High correlation was observed between the BDB-hemagglutination and the neutralization titer of exogenous insulin in mouse. On the other hand neutralization titer of exogenous insulin was independant. The antiserum immunized by BDB-method had extremely high BDB-hemagglutination titer, moderate neutralization titer of exogenous insulin in mouse, and no neutralizing activity of endogenous insulin in mouse, no correlation between the results of three methods was found. These discrepancies suggested that BDB conjugated insulin had few biological activity and that guinea pig antisera reacted in a different way two endogenous mouse and exogenous ox insulin.
- 1963-05-30