2.Glutathioneによるkinin効果の増大とその応用(第4回プラスマキニン研究会)
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概要
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Inhibiting effect of glutathione (GSH) on kininase in various tissues kinin effect potentiation of it were investigated. Kininase activity in various tissue homogenate (lung, kidney, intestine, pancreas, adrenals, testis, heart, spleen, liver, brain etc.) of rabbit was inhibited at the concentration above 10 μM. Fifty per cent inhibition of kininase activity in serum was at concentration of 60 μM. In contrast, the inhibition by cysteine and thioglycollate were at the concentration of 300 μM and that by o-phenan-throline was at that of 6 μM. Kinetic study showed that GSH inhibited the binding of kinin to kininase in competitive manner. Dose-response curve of bradykinin shifted to left in the presence of GSH. Phatmscological actions of kinin and kinin forming enzyme were potentiated; i. e., the hypotensive effect of bradykinin was strengthened by the pretreatment of GSH or their simultaneous administeration to the animal. Other kininase inhibitors such substances as o-phenanthroline, cystein and Ca-EDTA had almost no potenciative effect. There was , therefore, no parallelism between kinin effect potentiation and the inhibiting power for kininase. GSH was pharmacologically inctive unless kinin was present; i. e., the hypotensive effect of GSH appeared only in the condition that kallikrein was present in active state. When it was inactivated by Trasylol, GSH could not show any effect. Pharmacological actions of kinin such as vasodilatation, capillary permeability increment smooth muscle stmulation and blood pressure lowering were potentiated significantly by GSH and not by the other kininase inhibitors. GSH specifically modified the effect of kinin but not the effects of eledoisin, ATP, Ach, 5-HT and histamine. Accordingly, GSH can be used as a tool to determine whether kinin partcipiates in a certain physiological and pathological event in the body or not.
- 日本アレルギー学会の論文
- 1970-08-30
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