Studies on c-AMP Contents in Sea Urchin Eggs Fertllized wlth Normal and X Irradlated Sperm

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Intracellular levels of cyclic 3',5'-adenosine monophosphate (c-AMP) seemed to be constant through the first cleavage cycle of sea urchin eggs. X-irradiation to the sperm, which induced the first cleavage delay, did not change this level. Although it was shown in the previous paper that X-ray-induced cleavage delay was reduced by caffeine but not by aminophylline, both caffeine and aminophylline caused an increase in c-AMP levels. These results indicated a possibility that c-AMP does not mediate this caffeine effect on cleavage delay. Some recent works of radiation induced mitotic delay revealed that the progression of cell cycle through G2-phase or the release from G2-block is accelerated by caffeine treatment. Since caffeine inhibits c-AMP phosphodiesterase activity, which results in increase in intracellular c-AMP levels, it is suggested that c-AMP plays important role in G2-phase and G2-block. In sea urchin eggs, c-AMP is also shown to be present and its amount increases at fertilization. Further, c-AMP is demonstrated to be closely related to the cytokinesis of the cleavage cycle.
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