Effects of Physical and Chemical Agents on Competingand Transforming-Abilities of DNA in Bacillus subtilis

概要

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Ability of various DNA and biopolymers to compete with genetic marker-bearing DNA in the Bacillus subtilis transformation system was studied. Among tested polymers, only DNA exhibited the competing ability. Treatment of DNA with physical or chemical agents destroyed the competing ability. The competing ability of the treated DNA was compared with other abilities of the same DNA,i.e., transforming ability on another marker and ability to compete with ^3H-labeled marker DNA at incorporation into host cells. A parallelism was found between the transformation-competing ability and the incorporation-competing ability, while a parallelism was not necessarily hold between the competing ability and the transforming ability depending upon the agent used. From the modes of actions on these abilities of DNA, the agents were classified in two major groups, I) those which inactivate the trasforming ability of DNA, but exert little effect on its competing ability (e.g., ionizing radiation exerting indirect action, UV, nitrogen mustard, nitrous acid, 4 HAQO, DNase I, sonication) and II) those which inactivate the transforming ability of DNA as well as its competing ability (e.g., ionizing radiation exerting direct action, shearing). Nature of molecular damages caused by these agent was discussed.
日本放射線影響学会の論文