脳梗塞に関する実験的研究 : フリーズフラクチャーレプリカ(FFR)法による毛細血管透過性の超微形態学的検討

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概要

• 論文の詳細を見る

• Underlying mechanisms which lead to brain edema following ischemic insults have been subjects of much debate. In this study, experimental cerebral infarction was produced in dogs by injecting one or two silicone rubber cylinders through the cervical internal carotid artery. Twenty-four hours after embolization, animals were killed and morphometrical analyses in the capillary endothelial cell membranes in the cortex were done by freeze-fracture replica in addition to conventional ultra-thin section using the transmission electron microscope. In ultra-thin sections of capillary endothelium in the ischemic cerebral cortex, surface infolding and increase in number of pinocytotic vesicles were recognized, but no findings of opening of tight junction were ascertained. In freeze-fracture replicas of capillary endothelium of both normal and ischemic cerebral cortex, tight junction was presented as the network of ridges composed of about 100 Å particles on the protoplasmic face (PF) and grooves on the extracellular face (EF). In the ischemic cortex, arrangement of strands of these protein particles was not changed. In replica preparations, too, no definite findings of opening junction were certificated. Pinocytotic vesicles were seen as invaginations on the PF and as protrusions oh the EF. In the ischemic cortex, the average number per square μ/lm was increased, On the luminal front, it reached to 22.0 (on the PF) and 29.5 (on the EF) as compared to 7.2 and 9.0 in the normal cortex, respectively. The size of vesicles was also enlarged; 4,990.7±798 nm^2 (on the PF) and 4,762.8±878 nm^2 (on the EF) as compared to 3,567.7±570 nm^2 and 3,404.5±573 nm^2 in the normal cortex, respectively. These results indicate that transcellular transport by pinocytotic vesicles has an important role in increase of capillary permeability observed in an ischemic model.

• 日本脳神経外科学会の論文
• 1985-04-15

著者

• 井上 優

  井上脳神経外科

• 井上 優

  長崎労災病院脳神経外科

• 井上 優

  長崎大学脳神経外科

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