悪性黒色腫と有棘細胞癌の培養細胞株におけるケラチン線維の免疫電顕

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Heretofore, epithelial cells have been considered to be the only source of keratin (K) polypeptides that assemble into 10-nm filaments to form an extensive cytoplasmic network in conjunction with nuclear and cytoplasmic membranes. However, K was recently found to be expressed also in cultured non-epithelial normal and tumor cells: melanocyte, fibroblast, endothelial cell, malignant melanoma, fibrosarcoma, and angiosarcoma. Nevertheless, electron microscopy was incapable of detecting the K filaments (Katagata et al., J Dermatol Sci, 30, 1-9, 2002, see ref. 11). That is, K may present as subunits in each of the cultured cells named above, not as a filament formation. We used squamous cell carcinoma observed with immunoelectron microscopy, a more precise and conclusive technique, to further confirm whether or not K filament is formed in those cultured cells. HaCaT, an immortalizd keratinocyte cell line used as a positive control, yielded elegant immunoelectron microscopic images. Considerable K filament formations existed in malignant melanoma using anti-K or anti-vimentin antibodies, as revealed by the presence of linear immune gold particles on high electron density substances. In the case of squamous cell carcinoma, the gold particles were fewer than those of malignant melanoma. By contrast, no K filaments were detected in the other non-epithelial normal and tumor cell lines: fibroblast, endothelial cell, fibrosarcoma and angiosarcoma. These results suggest that the formation of K filaments in malignant melanoma (and slight presence in squamous cell carcinoma) is a particular and cell-dependent characterization.
山形大学の論文
2005-08-15

悪性黒色腫と有棘細胞癌の培養細胞株におけるケラチン線維の免疫電顕

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