マウス神経芽腫C-1300の生物学的性格に関する研究
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Murine C-1300 neuroblastoma has similar characters to human neuroblastoma and has been accepted as a model for investigation of human neuroblastoma. However the characters have not yet been clarified sufficiently. Establishment and characterization of the ascites form of C-1300 neuroblastoma were studied. Priming of the A/J mouse was performed with 0.5ml pristane nine days before implantation of the tumor. After dispersion of the C-1300 neuroblastoma cells, 1×10^6 cells were intraperitoneally inoculated. Ascites was accumlated after 13 days. It was serially transplantable with the ascites. The biological characteristics of the ascites tumor were as follows: 1. The ascites tumor cells appeared to the free cell type and were more anaplastic than those of the original solid tumor. 2. They maintained the same histopathological characters as the original solid tumor. 3. Tumor cell number of inoculated ascites could be completely controlled. Implantation rate was 100%, the survival days were almost constant. 4. Modal chromosome number of the ascites tumor cell was 93 with 8 maker chromosomes. 5. The generation time was about 22.3 hours by the BrdU-Acridine Orange method. 6. Research of cell kinetics and chromosome studies were easily performed. These results indicate that the C-1300 ascites tumor is a useful screening model for human neuroblastoma and may be applied for chemotherapy research and other cancer studies.
- 日本小児外科学会の論文
- 1983-10-20
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