INFLUENCE OF DI-(2-ETHYLHEXYL) PHTHALATE ON FETAL TESTICULAR DEVELOPMENT BY ORAL ADMINISTRATION TO PREGNANT RATS
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概要
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Influence of di-(2-ethylhexyl)phthalate (DEHP) on testicular development was studied by oral administration of DEHP at doses of 500 and 1000mg/kg/day to pregnant rats on gestational days (G) 7 to 18. Ethinyl estradiol (EE) at dose levels of 0.25 and 0.5mg/kg/day was used as a reference substance. Each 5-6 pregnant rats were sacrificed and their fetuses were examined on G12, 14, 16, 18 and 20. Fetal deaths averaging 20-36% were observed at every examination in the group receiving 1000mg/kg of DEHP. Increases of fetal deaths over 50% were also observed in the reference group that received 0.5mg/kg of EE. Microscopic examination of the fetal testis in groups treated with DEHP revealed degeneration of germ cells in G16 fetuses and localized proliferation or hyperplasia of interstitial cells in G18 and 20 fetuses. Germ cells having more than two nuclei were observed in a few cases including the control testes of G14 fetuses. These multinucleated cells were observed frequently in G20 fetuses treated with DEHP. Examination of testes of naturally delivered offspring of dams treated with 1000mg/kg of DEHP at 7 weeks of age revealed scattered atrophy or dilatation of seminiferous tubules. Another experiment was carried out to confirm the dose of DEHP affecting testicular development and spermatogenesis. DEHP was given to pregnant rats at doses of 125, 250 and 500mg/kg/day during G7-18. Similar histopathological changes were observed in fetal testes of the group exposed to 500 and 250mg/kg of DEHP, but not in those exposed to 125mg/kg. In postnatal examinations, however, no abnormality was found in the testes at 5 and 10 weeks after birth in any of the treated groups. Furthermore, no abnormal findings were observed in the function of sperm, sperm counts and sperm morphology in the offspring of the group treated with DEHP during the fetal period at 10 weeks of age. Thus, 125mg/kg/day is considered the no-observed-effect-level of DEHP on testicular development of rats by exposure in utero during the period of organogenesis.
- 日本トキシコロジー学会の論文
- 2005-08-29
著者
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齋藤 幸夫
慶応大理工
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Shirota Mariko
Hatano Research Institute, Food and Drug Safety Center
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Ono Hiroshi
Hatano Research Institute, Food and Drug Safety Center
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Shirota Mariko
Hatano Res. Inst. Food And Drug Safety Center
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Shirota M
Hatano Research Institute Food And Drug Safety Center
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HORIUCHI Shinji
Hatano Research Institute, Food and Drug Safety Center
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Saitoh Yuji
Department Of Material Physics Osaka University
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Saito Y
Keio Univ. Yokohama
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Saito Yahachi
Faculty Of Engineering Mie University
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斎藤 幸夫
慶応大学理工学部物理
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Shirota Mariko
Research Institute Of Biosciences And High-tech Research Center Azabu University
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SAITO Yoshiaki
Hatano Research Institute, Food and Drug Safety Center
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IMAI Kiyoshi
Hatano Research Institute, Food and Drug Safety Center
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YOSHIMURA Shinsuke
Hatano Research Institute, Food and Drug Safety Center
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SATO Masako
Hatano Research Institute, Food and Drug Safety Center
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NAGAO Tetsuji
Hatano Research Institute, Food and Drug Safety Center
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KATOH Masanobu
Mitsubishi Chemical Safety Institute Ltd
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Kato Masanobu
Mitsubishi Chemical Safety Institute Ltd
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Imai Kiyoshi
Hatano Research Institute Food And Drug Safety Center Laboratories Of Toxicology And Pathology
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Yoshimura Shinsuke
Hatano Research Institute Food And Drug Safety Center
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Kato M
Safety Study Department Nihon Bioresearch Inc.
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Nagao T
Hatano Research Institute Food And Drug Safety Center:(present Address)department Of Life Sciences F
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Nagao Tetsuji
Hatano Research Institute Food And Drug Safety Center
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Kato Michiyuki
Drug Safety Research Cehter Developmental Research Lab. Daiichi Pharmaceutical Co. Ltd.
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Kato Michiyuki
Drug Safety Research Center Developmental Research Laboratories Daiichi Pharmaceutical Co. Ltd.
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Saito Yoshiaki
Hatano Research Institute Food And Drug Safety Center
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Suwa Y
Research Center For Advanced Science And Technology The University Of Tokyo
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Kato Michiyuki
Drug Safety Research Center Daiichi Pharmaceutical Co. Ltd.
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Imai Kiyoshi
Dept. Of Toxicology And Pathology Hatano Research Institute Food And Drug Safety Center
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Horiuchi Shinji
Hatano Research Institute Food And Drug Safety Center
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Ono Hiroshi
Hatano Research Institute Food And Drug Safety Center
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Ono Hiroshi
Hatano Research Institute (hri) Food And Drug Safety Center (fdsc)
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Ono Hiroshi
School Of Veterinary Medicine Department Of Pharmacology
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Ono Hiroshi
Hatano Res. Inst.
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Sato Masako
Hatano Research Institute Food And Drug Safety Center
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Imai Kiyoshi
Hatano Research Institute Food And Drug Safety Center
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Imai Kyoshi
Hatano Research Institute Food And Drug Safety Center
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Imai Kiyoshi
Hatano Research Institiute Food And Drug Safety Center
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Nagao Tetsuji
近畿大学 発生生物学研究室
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Saito Yoshiaki
Hatano Research Institute (hri) Food And Drug Safety Center (fdsc)
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Kato Masashi
Department Of Bioenvironmental Medline Chiba Univ.:center For Environment Health And Field Sciences
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Horiuchi Shinji
Animal Care Hatano Res. Inst. Food And Drug Safety Center
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Saito Yoshiaki
Hatano Res. Inst. Food And Drug Safety Center
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NAGAO Tetsuji
Hatano Research Institute
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