INHIBITORY EFFECT OF ACEPHATE (N-ACETYL O, S-DIMETHYL THIOPHOSPHORAMIDE) ON SERUM CHOLINESTERASE : Effect of Acephate on Cholinesterase
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概要
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The Lineweaver-Burk plot of the activity of human serum cholinesterase against the concentration of butyrylthiocholineiodide was shown by two intersecting lines. The Hill plot of cholinesterase activity was linear over the entire range of the substrate concentration. The n value, an interaction coefficient, was less than 1.0 (about 0.8). These results suggest that cholinesterase has multiple substrate binding sites. Acephate, one of the organophosphorous insecticides, inhibited the activity of cholinesterase. Acephate at concentration under 1.25 mM (about 230 ppm in serum) did not inhibit the activity of cholinesterase. The minimum concentration of acephate inhibition of cholinesterase activity was at 2.5 mM. An equillibrium constant (K') can be used as an indicator of inhibitory effect on cholin esterase. The serum cholinesterase activity of workers who were exposed to acephate is not affected when the concentration of acephate in serum is less than 200ppm. This result suggests that the activity of serum cholinesterase is not an accurate indicator of the exposure of the low toxic insecticides, e.g. acephate. The inhibitory effect of acephate on cholinesterase decreased after the incubation with S-9 mixture. This result suggests that a part of acephate is metabolized to inactive substances in the liver.
- 日本トキシコロジー学会の論文
- 1982-08-25
著者
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Wakamatsu Kunimitsu
Department Of Biology Faculty Of Science Kyushu University
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Wakamatsu Kunimitsu
Department Of Biology Fukuoka Women's University
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Ando Mitsuru
Environmental Health Sciences Division National Institute For Environmental Studies
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- INHIBITORY EFFECT OF ACEPHATE (N-ACETYL O, S-DIMETHYL THIOPHOSPHORAMIDE) ON SERUM CHOLINESTERASE : Effect of Acephate on Cholinesterase