Isolation of Transposon Tn5-Induced Hydrophobic Mutants of a Bradyrhizobium japonicum Strain with Improved Competitive Nodulation Abilities

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概要

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• Hydrophobic mutants of the Bradyrhizobium japonicum strain 138NR were obtained by transposon Tn5 mutagenesis followed by replica-plating on polystyrene plates. Fifteen mutants were isolated at a frequency of 10^<-6>. Gel-filtration analysis of the exopolysaccharides revealed that the hydrophobic mutants produced a significantly smaller amount of low-molecular-weight polysaccharides than the parent. Four of the isolated mutants formed a larger number of nodules on soybean (Glycine max L. Merr. cv. Tamahomare) than the parent, two were superior in symbiotic nitrogen fixation, and two were symbiotically defective on soybean. Competitive nodulation abilities of the mutants were examined by inoculating them to soybean with B. japonicum strain 123ET as a competitor and determining the nodule occupancy based on the antibiotic resistance. All the mutants tested except for the symbiotically defective ones were superior in their competitive nodulation ability to the parent strain. When inoculated 24 h before the inoculation with 123ET, the mutants exhibited an increased nodule occupancy (44-93% compared to 9% by the parent).

• 社団法人日本土壌肥料学会の論文

著者

• OGATA Hiroshi

  Department of Physics, Faculty of Science, Kyoto University

• Ozawa Takashi

  Department of Applied Biochemistry, College of Agriculture, Osaka Prefecture University

• Ogata Hiroshi

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka

• Doi Ryouichi

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka

• Komai Yutaka

  Department of Agricultural Chemistry, College of Agriculture, University of Osaka

• Doi Ryouichi

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka

• Komai Yutaka

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka

• Ozawa Takashi

  Department Of Agricultural Chemistry College Of Agriculture University Of Osaka

• Ozawa Takashi

  Departmant Of Chemistry Faculty Of Engineering Shibaura Institute Of Technology

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