Activity of Protease Extacted from Rice-Rhizosphere Soils under Double Cropping of Rice and Waeat

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概要

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• Protease-active extracts were prepared from 2 rice-rhizosphere and non-rhizosphere soil samples 3 proteolytic isolates from the soil samples which were taken from water logged paddy fields(Gray Lowland Soils)under double cropping of rice and wheat that had benn treated with chemical fertilizer(CF)or organic manure(OM). The activities were assayed and characterized using an artificial substrate, benzyloxycarbonyl-L-phenylanyl-L-tyrosyl-L-leucine(ZFTL). The protease-active extracts from the rhizosphere and non-rhizosphere of the CF and OM soils(abbreviated CFR, CFN, OMR, and OMN)shoewed an optimum pH between 7 and 8. The extracts hydorlyzed ZFTL to Tyr-Leu and Leu ; generally, Tyr-Leu was the main reaction product. Activities of all the samples were inhibited by EDTA and p-chloromercuribenzoic acid(PCMB)but not by pepstatin and phenylmethanesulfony fluoride(PMSF). These results indicate that the main components of the ZFTL-hydrolases in CFR, CFN, OMR, and OMN were essentially similar. Optimum pH for the activity of the protease extracted from a Gram negative rod(MNB003)and two actinomycetous isolates(MNA014 and MRA017) was about 8, 8, and 8.5, respectively. Extract from the culture of MNB003 hydrolyzed ZFTL to Tyr-Leu and Leu, and the protease activity was inhibited by EDTA and PCMB. Protease activities of the extracts from cultures of the two actinomycetes were inhibited by EDTA but not by PCMB. The extact from culture of MNA014 produced mainly Tyr-Leu from ZFTL while MRA017 produced Leu. Characteristics of the protease activity from cultures of MNB003 and MNA014 were similar to those of the enzyme extracted from a paddy field soil under single cropping(Takeuchi and Hayano 1994 : Soil Sci.Plant Nutr., 40, 691-695)and from an Andosol under tomato cultivation(Hayano et al. 1987 : Biol. Fertil. Soils, 4, 179-183), respectively.

• 社団法人日本土壌肥料学会の論文

著者

• Asakawa S

  Kyushu National Agricultural Experiment Station Kumamoto Jpn

• Hayano koichi

  Kyushu National Agricultural Experiment Station

• Watanabe Katsuji

  Kyushu National Agricultural Experiment Station

• Asakawa Susumu

  Kyushu National Agricultural Experiment Station

• Hayano K

  Kyushu National Agricultural Experiment Station : (present Address)national Institute Of Agro-enviro

関連論文

• Protease Activity in a Paddy Field Soil : Origin and Some Properties
• Activity of Protease Extacted from Rice-Rhizosphere Soils under Double Cropping of Rice and Waeat
• Source of Soil Protease Based on the Splitting Sites of a Polypeptide

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