原子間力顕微鏡によるDNA損傷の検出 : 第28回大会シンポジウム : II.新しい変異原検出法へのアプローチ

概要

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An atomic force microscope (AFM) has become a useful tool to visualize biological molecules with nanometer level resolution. It is theoretically possible to visualize biological molecule in the original form by AFM. Many types of biological molecules, such as DNA and protein, have been observed in air or in water by AFM. We used AFM for nanometer-level structural analysis of chromosome damage induced by radiation. The AFM could image the structure of chromosomes on Giemsa stained or unstained samples. Atomic force microscopy to analyze DNA structure was also introduced to further explain of formational changes in plasmid DNA after radiation exposure in comparison with agarose gel electrophoretic analysis (Murakami, M., et al., 2000). Three forms of plasmid DNA, closed circular (intact DNA), open circular (DNA with single strand break) and linear form (DNA with double strand break) were visualized by dynamic force mode AFM after γ-irradiation. The torsional feature of the plasmid DNA was imaged better with AFM than with a transmission electron microscope (TEM).
日本環境変異原学会の論文
2000-07-31