サルモネラTA102およびTA104株を用いた活性酸素生産系の変異原性に及ぼす抗酸化剤の影響

概要

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The effects of antioxidants on the mutagenicity of reactive oxygen species (ROS) was examined using the Ames test with Salmonella typhimurium TA102 and TA104 tester strains. In this study, adriamycin, t-butyl hydroperoxide (BHP) , and H_2O_2 were used as generating systems of oxygen-derived radicals. Also, ascorbic acid, thiourea, and mannitol were added as the antioxidants. The number of adriamycin-, BHP-, and H_2O_2-induced revertant colonies of TA102 and TA104 showed dose-related increases. Then, ascorbic acid reduced adriamycin-and BHP-induced reversion in TA102, and reduced adriamycin-, BHP-, and H_2O_2-induced reversions in TA104. Thiourea reduced the number of revertant TA102 colonies induced in all 3 ROS-generating systems, and reduced BHP-and H_2O_2-induced reversion in TA104. Mannitol reduced adriamycin-induced reversion in TA102. These results not only indicate that this test system using TA102 and TA104 tester strains can detect the mutagenicity of active oxygen, but also suggest that this test system could be used to screen for the antimutagenicity of antioxidants which suppress the oxidative damage of DNA by scavenging active oxygen.
日本環境変異原学会の論文
2000-02-28