コメットアッセイを用いたDNA損傷の検出 : 試験原理の検証 : シンポジウム : III. コメット法, その変異原性試験としての展望

概要

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The comet assay utilizes some of the chemical properties of DNA. DNA is denatured and unwound at pH generally above 12.0. Furthermore, at pH 12.6 or higher alkaline condition, alkali labile sites are quickly transformed to strand breaks. Thus DNA with strand breaks and alkali labile sites becomes shorter single strand DNA at about pH 12.0 and 12.6 or higher, respectively. Therefore the alkaline condition is an important factor for the comet assay to detect the genotoxic { effects of chemicals. In order to verify the relationship between DNA migration and alkaline condition, the effect of model mutagens with different mode of action was evaluated with the ~ comet assay under two different alkaline conditions (pH12.1 and 12.6) . We also evaluated the relationship between the onset of DNA damage and the characteristics of mutagens. The results of these studies demonstrate that the comet assay will provide reliable data based on the test principles and biological response. (This paper, in a session chaired by Yuu Sasaki and Takaji lkushima, was presented to the symposium "A view of Comet assay as a mutagenicity test system", organized by Yuu Sasaki and Takaji lkushima, at the 27'" annual meeting of the Environmental Mutagen Society of Japan, and held at the Mielparque Osaka in Osaka, Japan, November, 24-26, 1998.)
日本環境変異原学会の論文
1999-07-12