異なる温度でのシオミズツボワムシBrachionus plicatilis Mullerの配偶者認知

概要

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Recent studies on sexual isolation among euryhaline Brachionus species suggest that differences in the molecular structure of a mate recognition pheromone (MRP) are important in maintaining reproductive isolation. It has not been clarified whether environmental conditions can affect MRP structure and the responsiveness. The objective of this study was to examine the effect of temperature on mate recognition of the rotifer B. plicatilis. Three strains (Russian, German, and Tokyo) of B. plicatilis were cultured on Nannochloropsis oculata and a series of intra- and interstrain matings were made using female and male rotifers reared at three temperatures, 15, 20 and 25℃. Matings were conducted for nine combinations of treatments (3 strains x 3 temperatures). Temperature affected the attempted matings (frequency of circling behavior per male/female contact) differently among strains and sexes. Culture temperature of females affected the frequency of mating attempts. Male recognition of females occurred best at 20℃ for Russian strain. German strain males only recognized females when females were reared at less than 20℃. For Tokyo strain, however, rearing temperature of females did not affect mate recognition. MRP antibody binding did not correlate with the frequency of male mating attempts at each temperature.