ダイズシストセンチュウ卵の前処理と根浸出液のふ化促進効果の関係

概要

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Soil samples containing cysts of the soybean cyst nematode, Heterodera glycines ICHINOHE, were taken from a field of soybean plants. They were stored in sealed vinylon bags at 5℃ and the initial moisture was maintained all through storage. Just before the experiments, the cysts were collected from the stored soil samples, and the eggs were freed from them. Five series of experiments were conducted to investigate the effect of combinations of the three factors, namely the temperature of kidney bean root diffusate, temperature of pre-soaking water and pre-soaking period on the hatching of free eggs. The eggs were pre-soaked in water at constant temperatures of 5,10,15,20,25,30 or 35℃, for various periods up to 100 days, and soaked in the root diffusate at constant temperatures of 20,25 or 30℃. The rate of hatching from the eggs reached the highest when the eggs were presoaked in water at 25℃ or 30℃ for 1 to 4 weeks and soaked in the root diffusate at 25℃. The hatching rate in water, however, was extremely high when pre-soaked at 25℃ than at 30℃, so that the hatching rate in the diffusate became lower at 25℃ than at 30℃. Therefore, it is a suitable method to pre-soak in water at 30℃ for obtaining larvae of a relatively uniform stage during a short period in the diffusate at 25℃. One week was found to be enough as pre-soaking period when conducted at 30℃.
日本応用動物昆虫学会の論文
1971-12-25