Discrimination among pest species of Bactrocera (Diptera : Tephritidae) based on PCR-RFLP of the mitochondrial DNA

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概要

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• A PCR-RFLP based method of species identification was considered for 18 pest species of Bactrocera fruit flies mainly distributed in the Asia-Pacific region. A 1.6kb portion of the mitochondrial DNA containing portions of 16S and 12S ribosomal RNA genes was determined for eight species for which the mtDNA sequences have not been studied. Comparisons of restriction patterns expected from the sequences obtained in this and previous studies revealed that all 18 species can be discriminated by PCR-RFLP using a small number of restriction enzymes. Based on the highly conserved sequences detected among the 18 species, four sets of PCR primers were designed to amplify shorter, diagnostically informative sections within the mtDNA fragment. Given the simplicity of banding patterns and the ease of amplification of DNA fragments, such sections were considered suitable for PCR-RFLP analysis. Based on the results, a scheme for the Bactrocera pest species identification was proposed. The PCR-RFLP analysis using 83 individuals revealed that the scheme correctly identified most of the 18 species except for two closely related sympatric species, Bactrocera carambolae and B. papayae; the majority of individuals of the former species showed the same banding patterns as the latter species. In addition, molecular phylogenetic analyses were performed based on the nucleotide sequences in order to examine the positioning of each species among many other species of fruit flies.

• 日本応用動物昆虫学会の論文
• 2002-08-25

著者

• MURAJI Masahiko

  National Institute of Agrobiological Sciences

• Muraji M

  National Institute Of Agrobiological Sciences

• NAKAHARA Shigehito

  Yokohama Plant Protection Station

• Nakahara Shigehito

  Yokohama Plant Protection Station:(present Address)naha Plant Protection Office

関連論文

• Genetic divergence among populations of the white grub beetle, Dasylepida ishigakiensis (Coleoptera: Scarabaeidae), distributed in the southern part of the Ryukyu Islands of Japan, detected from mitochondrial DNA sequences
• Genetic variation of the green chafer, Anomala albopilosa (Hope) (Coleoptera: Scarabaeidae), in the Ryukyu Islands of Japan detected by mitochondrial DNA sequences
• Relationship among local populations of the white grub beetle, Dasylepida ishigakiensis (Coleoptera: Scarabaeidae), detected by phylogenetic analysis based on the mitochondrial DNA sequence
• Genetic variations among and within populations of the Oriental fruit fly, Bactrocera dorsalis (Diptera; Tephritidae), detected by PCR-RFLP of the mitochondrial control region
• Discrimination among pest species of Bactrocera (Diptera : Tephritidae) based on PCR-RFLP of the mitochondrial DNA
• The Philippines is a possible source of the Bactrocera dorsalis complex species (Diptera, Tephritidae) occasionally collected in the Ryukyu Islands of Japan; analyses of mitochondrial DNA

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