STUDIES ON TOXOPLASMOSIS V : COMPLEMENTAL OBSERVATIONS ON THE TISSUE CULTURE METHOD, ESPECIALLY ON THE EFFECT OF THE NUTRIENT FLUID UPON THE INVASION AND MULTIPLICATION OF THE ORGANISMS
スポンサーリンク
概要
- 論文の詳細を見る
Following the previous report, complemental observations were carried out to ascertain the suitable cultural conditions including the effects of the nutrient fluid upon the invasion and multiplication, the ultimate aim being to promote the utility of tissue culture in isolation of the organisms. Throughout the experiments HeLa cells and T. gondii strain RH were used. Data obtained are summarized as follows : 1. Good invasion occured very frequently when Hanks' balanced salt solution and solutions devoid of divalent ions such as Mg, Ca, were employed. However, there seems to be no significant differences in the ratio of invasion due to the culture fluids tested-H (Hanks), HL (Hanks plus lactalbumin hydrolysate), HLS (HL plus serum), YLE (yeast extract, lactalbumin hydrolysate and Earle's balanced salt solution) and YLES (YLE plus serum), and organism numbers more than 90% may invade the host cell 24 hours after inoculation (fig. 1). 2. The organisms which were suspended in the culture fluid devoid of protein substances tend to die in rather short period and could not multiply well in cells when cultured by these proteinless nutrients (Fig. 3 A, Table 1). Accordingly, the nutrients must be replaced by ones containing protein after 5〜7 hours when the media such as Hanks were employed in inoculation (Fig. 3B). 3. Addition of bovine sera to the concentration of 0.5〜1.0% seems to be quite enough to cause heavy growth of T. gondii if the medium HL is used for the culture nutrient. 4. PH values ranging from 6.5 to 8.0 do seem not to show any effects on the invasion ratio of the organisms. However, in the more acid or alkaline sides (6.0,8.0) poor multiplication was always observed and especially in acid side, elution of the soluble complement-fixing antigenic substances was also scarce (Fig. 2). 5. The volume of the suspension of the inocula seemed to have no significant relations with the yield of the organisms when the inoculated bottles had been left overnight for the invasion (Fig. 7). 6. To cultivate a small number of the organisms in material, frequent changes of the nutrient fluid every day or every other day during the first week are very necessary (Fig. 6). 7. Clear proofs of the multiplication of the organism numbers of 2.5～5.0×10^3 in the materials were observed by counting method when the tests were performed with due consideration of the above described points (Fig. 8).
- 北海道大学の論文
著者
-
SHIMIZU K.
Department of Material Physics, Faculty of Engineering Science, Osaka University
-
Shimizu Kiheiji
Department Of Hygiene And Microbiology Faculty Of Veterinary Medicine Hokkaido University
-
Shimizu Kiheiji
Department Of Hygiene And Microbiology Faculty Of Veterinary Medicine Hokkaido University:(present A
関連論文
- Oxygen under High Pressure-Temperature Dependence of Electrical Resistance
- STUDIES ON HEPATITIS CONTAGIOSA CANIS I. : INFECTION EXPERIMENTS ON DOGS WITH TWO STRAINS OF THE VIRUS AND SEROLOGICAL INVESTIGATIONS WITH THE COMPLEMENT-FIXATION TEST
- Studies on the Myocardial Metabolism in Diabetes Mellitus : THE EFFECT OF INSULIN ON MYOCARDIAL CARBOHYDRATE METABOLISM(Proceedings of the 24th Annual Meeting The Japanese Circulation Society [Part II])
- ELECTRON MICROSCOPIC STUDY ON THE DEVELOPMENT OF FLAGELLA OF A FRESHLY ISOLATED STRAIN OF SALMONELLA ABORTUS EQUI
- Epidemiological Study of Maternal and Environmental Factors Associated with Early Intrauterine Death of Human Embryos : Poster Sessions
- STUDIES ON TOXOPLASMOSIS II. : SOME OBSERVATIONS ON STRAIN "HT" WHICH WAS ISOLATED FROM A HARE (LEPUS TIMIDUS AINU) IN SAPPORO
- BACTERIOLOGICAL STUDIES ON STREPTOCOCCI FROM BOVINE UDDER II. : DISTRIBUTION OF STR. AGALACTIAE IN DOMESTIC ANIMALS AND LONG-TERM OBSERVATIONS ON THE UDDER WHICH HARBOURED THE ORGANISM
- BACTERIOLOGICAL STUDIES ON STREPTOCOCCI FROM BOVINE UDDER I. : SEROLOGICAL AND BIOCHEMICAL OBSERVATIONS ON GROUP-B STREPTOCOCCI AND A GENERAL DESCRIPTION OF STREPTOCOCCI FROM BOVINE MILK IN HOKKAIDO
- STUDIES ON TOXOPLASMOSIS V : COMPLEMENTAL OBSERVATIONS ON THE TISSUE CULTURE METHOD, ESPECIALLY ON THE EFFECT OF THE NUTRIENT FLUID UPON THE INVASION AND MULTIPLICATION OF THE ORGANISMS
- STUDIES ON TOXOPLASMOSIS I : AN OUTBREAK OF TOXOPLASMOSIS AMONG HARES (LEPUS TIMIDUS AINU) IN SAPPORO
- Modification of the Drain Current on a Metal-Oxide-Semiconductor Field-Effect Transistor with Ferrite Gate Oxide
- VECTORCARDIOGRAPHIC STUDY OF LEFT VENTRICULAR PRESSURE LOAD : DIFFERENCE OF QRS VECTOR BETWEEN ESSENTIAL HYPERTENSION AND AORTIC STENOSIS : The Fifth Conference on Vectorcardiography
- Expression of Cholecystokinin in the Pancreas During Development
- Dermatological manifestations in Yusho : correlation between skin symptoms and blood levels of dioxins, such as polychlorinated dibenzofurans (PCDFs) and polychlorinated biphenyls (PCBs)
- LOCAL INTRA-ARTERIAL THROMBOLYSIS IN HYPERACUTE CEREBRAL EMBOLISM. ITS VALIDITY AND LIMITATIONS
- CA Nerve Endings in the Rat Preoptic Area by Fluorescence Histochemistry and Electron Microscopy.
- Magnetic Properties of LuVO_3
- Effects of Hydrostatic Pressure on Martensitic Transformations
- Quantitative Trait Loci on Chromosomes 10 and 11 Influencing Mandible Size of SMXA RI Mouse Strains