<原著論文>半定量的RT-PCR法を用いたマウス網膜における抗原提示機能の可能性と解析

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To identify the possibility of antigen presentation in the retina, westudied the production of various cytokines and the expression of cell surface moleculesin the retina by Reverse Transcription-Polymerase Chain Reaction (RT-PCR).Lipopolysaccharide (LPS) or inactivated Bordetella pertussis (BP) was injected into thevitreous cavity of C57BL/6J mice. The retinas were collected 3,6 and 24 hours after theinjection. Expression of mRNA for MHC class II, CDllc, CDSO, CDS6, toll-like receptor(TLR) 4, TLR9, IL-12p35, IL-2, IFN-y, IL-4, IL-5, IL-6, IL-IO, IL-lj3, TNF-a andTGF-j3 was analyzed by semi-quantitative RT-PCR. Expression of mRNA for MHCclass II, CDllc, CD80, CDS6, TLR4 and TLR9 was found in the untreated retina. Theamount of expression of MHC class II, CDllc, CDSO and CD86 increased after injectionof either LPS or BP. Expression of TLR4 mRNA increased only following injectionwith LPS, whereas TLR9 increased following injection with BP or LPS, not clear. Theexpression of cytokines, IL-12p35, IL-2, IFN-y, IL-4, IL-5, IL-6, IL-IO, IL-lj3, TNF-aand TGF-j3 increased after injection with either LPS or BP. A time course of mRNAexpression indicated a peak at 3 hours after injection, which then gradually subsided.These results suggest that proinflammatory cytokines related to antigen presentationwere upregulated by stimulation of TLR4 and TLR9.
秋田大学の論文