海外留学研究報告 Identification of ligands for functional Mn in the photosynthetic water-oxidizing complex.--An approach by combined techniques of photoactivation and chemical modification with amino group-labeling reagents
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概要
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Four Mn atoms function as catalysts in the photosynthetic water-oxidizing complex located on the PS II oxidizing side. We have studied the involvement of amino groups of the PS II proteins in photoligation of Mn^<2+> to the apo-water-oxidizing complex, using the combined techniques of photoactivation and chemical modification with the modifiers methyl acetimidate (MAI), acetic acid N-hydroxysuccimide ester (NHS) and 2,4,6-trinitro-benzenesulfonic acid (TNBS). Chemical modification of hydroxylamine-treated PS II core complexes decreased their capacity for restoration of oxygen evolution and photoligation of Mn^<2+> to the apo-water-oxidizing complex (WOC), but did not affect their activities of electron transfer in the vicinity of PS II. The number of functional high-affinity Mn-binding sites, but not of low-affinity sites, was significantly modulated by chemical modification. Kinetic analysis of photoactivation with the repetitive flashes revealed that the intermediate generated during a photoactivation process was destabilized by the chemical modification. To identify which proteins possess the amino groups involved in ligation of functional Mn, we examined the difference in NHS-biotinylation between PS II core complexes with and without the Mn cluster. NHS-biotinylation that altered the ligation of functional Mn apparently occurred on three proteins : an antenna-chlorophyll binding protein CP47,a light-harvesting chlorophyll protein CP29 and another chlorophyll binding protein PS II-S. Of these proteins, only the Mn-dependent biotinylation of CP47 was found to be independent of the NHS-masking concentration applied before removal of the function Mn. These results suggest that lysyl residues of CP47,and perhaps also CP29 and PS II-S, function in direct photoligation of Mn^<2+> to the apo-WOC.
- 福岡女子大学の論文
- 2002-03-20
著者
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Otsubo Mayuko
Laboratory Of Plant Physiology Faculty Of Human Environmental Sciences Fukuoka Women's Universi
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Otsubo Mayuko
Laboratory Of Horticultural Science Department Of Plant Resources Division Of Bioresource And Bioenv
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Theg Steven
Section Of Plant Biology Division Of Biological Sciences University Of California
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Tamura Noriaki
Laboratory Of Plant Physiology Fukuoka Women's University
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Tamura Noriaki
Laboratory Of Plant Physiology Faculty Of Human Environmental Sciences Fukuoka Women's Universi
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Ishikawa Yasuo
Laboratory Of Plant Physiology Faculty Of Human Environmental Sciences Fukuoka Women's Universi
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YAMAMOTO Yasusi
Department of Biology, Faculty of Science, Okayama University
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Yamamoto Yasusi
Department Of Biology Faculty Of Science Okayama University
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Yamamoto Yasusi
Department Of Biology Faculty Of Science Kyushu University
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田村 典明
Laboratory of Plant Physiology, Faculty of Human Environmental Sciences, Fukuoka Women's University
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