<研究報告>共焦点レーザー顕微鏡法を用いた両生類胚の細胞分裂の観察
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In order to investigate the molecular mechanisms of cell division in early development of amphibians, the embryos were fixed and stained with anti-cyclin B1 antibody or anti-α and γ tubulin antibodies and the second antibody conjugated with FITC. Cyclin B1 was localized in the condensed chromatins, chromosomes, centrosomes, and spindles. γ tubulin was localized centrosomes and poles of spindles. The confocal laser microscopy enabled us to observe the localization of these molecules in a large size of cells, more than 500μm in diameter.