クロショウジョウバエ・αGPDHs-アロザイムcDNAの塩基配列とそのアミノ酸変異

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Three cDNA clones coding for a variant αGPDH^<S-> enzyme of glycerol-3-phosphate dehydrogenase in Drosophila virilis were isolated by screening an adult αGPDH^<S-> cDNA library constructed in the λgt10 with αGPDH^M cDNA probe from the same species. The nucleotide and deduced amino acid sequences of these clones (a, b and c) were compared with those of αGPDH^M cDNA and αGPDH genomic DNA. The sequences of the coding region and 5' and 3' flanking regions of the a clone showed a high homology to those of αGPDH^M cDNA ; only a single substitution was found at 100 bp in the coding region, two substitutions at 1101 and 1115 bps in the 3' flanking, among the total 1560 bps. However, the single nucleotide substitution in the coding region resulted in a charge change of the amino acid from basic to acidic. The b clone was the same as the a clone except a long 3' flanking tail. This is caused by using the fourth polyadenylation signal out of the five signals in the 3' flanking region, while the a clone uses the first signal. These fact means that each signal can be randomly used when several polyadenylation signals are present in the 3' flanking sequences. In the case of the c clone, the sequences of the 5' flanking and the region encoding 1-349 amino acids were identical to those of the a clone, but afterward, 30 nucleotides encoding 10 amino acids, the TAA stop codon and the flanking sequences containing a polyadenylation signal follow, which sequences can not be found in the a clone. On the other hand, the a clone has the sequences coding for 350-352 amino acids and its 3' flanking region. It is apparent that the a clone is the transcript of exons 1-6 and exon 8,and the c clone, of exons 1-7 compared with the αGPDH genomic sequences. Such a differential splicing of the primarily transcriptional products was discussed.
城西大学の論文

クロショウジョウバエ・αGPDHs-アロザイムcDNAの塩基配列とそのアミノ酸変異

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