<原著>Endogenous Oxygen Radical Scavengerによるマウス退縮型癌細胞の悪性化進展の抑制に関する研究
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概要
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QR-32 cell is low tumorigemc and low metastatic clone derived from 3-methy cholanthrene induced C57BL/6 mouse fibrosarcoma (BMT-11). When QR-32 cells (2×10^5) were sub-cutaneously implanted or 10^6 cells were intravenously injected into normal mice, they all spontaneously regressed. However, when they were subcutaneously co-implanted with gelatin sponge (10×5×3mm), they acquired enhancement of tumorigenicity and metastatic ability and grew lethally. Previous in vitro assays have shown that this malignant progression of QR-32 cells was caused by oxygen radicals released from host cells reactive to gelatin sponge To elucidate the effects of oxygen radicals in vivo, this study examined whether endogeous oxygen radical scavengers induced by bismuth submtrate (BSN) or PSK administration inhibited the malignant progression of QR-32 cells. The QR-32 (2×10^5) cells and gelatin sponge were subcutaneously co-implanted into mice, pre-administered with BSN (5mg/kg/day) or PSK (150mg/kg/day, i p, 1000mg/kg/day p o). The BSN and PSK administration did not affect the tumor growth. The culture lines from these tumors arising in mice were established implanted with QR-32 cells and gelatin sponge, and implanted these culture tumor cells s c or i v into normal mice. Compared with the parental QR-32 cells, the tumor cell lines developed in the normal mice and used for the malignant progression estimation , i e s. c tumorigenicity and i v lung metastasis. All 18 cell lines (100%) obtained from the untreated group showed progress in the malignancy when compared with QR-32 cells However, these cell lines obtained from the BSN administered group indicated malignant progression in only 4 out of 7 cell lines (57.1%), in the same manner cell lines obtained from the PSK (i p) group indicated malignant progression in 3 out of 6 cell lines (50%), for cell lines obtained from PSK (p o ) group indicated in 4 out of 8 cell lines (50%). Endogeous radical scavengers in tumor in mice administered with BSN or PSK were analysed with the immunoblotting and immunohistostaming. These results of the assay showed that the quantity of radical scavengers (metallothionein, Mn-SOD, Cu/Zn-SOD GSH-Px, Catalase) increased over the untreted groups. These findings suggest that oxygen radicals may play an important role in in vivo QR-32 malignancy progress.
- 1994-12-31
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