Kinetics of Changes in Abundance of Transcripts Encoding Cytokines, Fas, and Fas Ligand in Susceptible and Resistant Mice Infected with Towplasma gondii

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The kinetics of changes in the abundance of mRMAs emcoding various cytokines (interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), and (IL-6)) and Fas, Fas ligand were measured by reverse transcription-PCR at various sites in both susceptible C57BL/6 and resistant BALB/c mice infected with the Fukaya Toxoplasma gondii (T. gondii). The abundance of transcripts encoding IFN-γ, TNF-α, and IL-6 increased markedly from two weeks after infection, reaching a plateau at four weeks, in the brains of C57BL/6 mice (p<0.05). In spleen, the abundance of IFN-γ and TNF-α mRNAs was increased two weeks after infection and then decreased in both mouse strains. Neutralization of endogenous IFN-γ markedly increased parasite number in the brain of C57BL/6 mice, whereas injection of exogenous IFN-γ had no effect. This observation indicates that endogenous IFN-γ is essential but not sufficient for inhibition of T. gondii growth in vivo. No changes in the abundance of IL-4 mRNA were observed in the brain or spleen of either mouse strains. The abundance of Fas mRNA was slightly higher (p<0.05) in the resistant BALB/c mice than in the susceptible C57BL/6 mice and the abundance of Fas ligand mRNA was slightly higher (p<0.05) in the susceptible C57BL/6 mice than in the resistant BALB/c mice. This observation suggests that Fas may contribute to a protective role in resistant BALB/c mice and that increased Fas ligand expression in brain of susceptible C57BL/6 mice may result from inflammation of toxoplasmic encephalitis.
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Kinetics of Changes in Abundance of Transcripts Encoding Cytokines, Fas, and Fas Ligand in Susceptible and Resistant Mice Infected with Towplasma gondii

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