馬流産菌の死菌免疫に關する研究 : I. 馬流産菌のマウスに對する病原性に就て

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In the present paper some experiments are described on the comparative studies on mouse virulence of the freshly isolated strains of this bacillus and of stock cultures stored for a period of 1∿26 years. The authors also investigated the spread of the organism by the various routes of administration of the bacilli. Besides, the effect of mucin on mouse virulence was tested. The results are summarized as follows : 1. The death-rate following intraperitoneal inoculation is affected by the weights of the mice. The heavier mice show a tendency to have stronger resistance. No difference between the two sexes is detectable (Tables 2,3). 2. The mouse virulence of the bacilli is to be estimated preferably by reading off the (LD)_<50> from the observed mortalities in each of the mice groups inoculated intraperitoneally with a series of six dilutions (10)^<-1>∿(10)^<-6>mg within 21 days. 3. The (LD)_<50> of each 10 freshly isolated strains and stock cultures were compared. Except in certain strains, there is no marked difference between the fresh and stock strains (Table 4). 4. The fresh strains isolated from pyogenic or inflammatory lesions appeared to be more virulent than the ordinary fresh strains from aborted fetus (Table 4). 5. Some biological and serological characters of 5 stock cultures preserved for more than ten years were observed, and the resistance to fresh guinea pig serum of these strains is markedly less than that of fresh strains. However, (LD)_<50> of two stock cultures are almost the same as fresh strains and three of them showed lower virulence (Tables 4,8). 6. The behavior of invasion and multiplication of the bacilli in mice administered by various routes of inoculation was tested. Large doses of bacilli administered intracerebrally or intraperitoneally cause deaths by the first septicaemia in almost all mice; subcutaneous injections also cause a lesser percentage of deaths. A minute dose of bacilli given by above 3 routes or a large dose such as 0.5∿1mg administered perorally or pernasally does not cause first septicaemia but secondary septicaemic infection (Tables 10∿18). 7. However, mice which suffered from secondary septicaemia do not necessarily die. Thus the mouse virulence of abortion bacilli, as many authors already stated, seems to be intermediate between Salm. typhi-murium and Salm. typhi. 8. No differences between the behavior of the invasion into mice of the fresh and stock cultures have been detected clearly with various routes of infection. It must be noted, however, that a minute dose of a fresh strain given by subcutaneous routes showed comparatively a wider spread of bacilli than that of a stock strain after 6 days but not after 2 weeks. 9. Even a small number of bacilli injection with mucin inevitably cause death of mice within a short period (Table 19). 10. The bacilli injected intraperitoneally with mucin multiply readily in the peritoneal cavity and invade the blood stream very soon (Tables 20,21). 11. The presence of mucin also enhances the virulence of a low-virulent strain and rough variant in a restricted degree. The enhancement of virulence goes parallel with its original virulence.
北海道大学の論文

馬流産菌の死菌免疫に關する研究 : I. 馬流産菌のマウスに對する病原性に就て

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