<原著>実験気管支喘息モデルにおけるβ-2-adrenergic receptorのmRNAの検討
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Changes in mRNA of the β-2-adrenergic receptor (β-2-receptor) could not be measured directly before. Using in- situ hybridization with an ^<35>S labeled DNA probe, encoding the gene of β-2 receptor, the localization of mRNA of β-2 receptor in the lung was determined by light microscopic autoradiography and the relationship between changes in mRNA and pulmonary function of animals at immediate asthmatic response (IAR) was assessed. To help clarify the localization and changes in mRNA, an experimental model of bronchial asthma was prepared. The first group of animals was sensitized to ovalbumin (OA) (OA 2). The second group of animals was killed at IAR (BA 1). The third group of animals was killed at 3 day after three-times inhalation of OA (BA 3). The fourth group of animals was killed after repeated exposure of nebulized OA (BA 12). Pulmonary function was assessed by % changes in tidal volume (V_T) before killing. The frozen section of these animals was mounted on cover-slips and then in- situ hybridization was done at 37℃ for 16 hours. Autoradiograms prepared by apposition of dry emulsion- coated coverslips showed that the distribution of mRNA of the β-2 receptor was wide spread in the lung. The alveolar epithelium was strongly labeled, but the smooth muscle and epithelium of bronchus were labeled weakly. The two types of reaction in % changes in V_T after OA inhalation were observed in BA 3. One was without IAR and another was rapid recovery from IAR after inhalation of OA compared with BA 1. These findings suggest that the β-2 receptor is regulated directly by mRNA in the lung. Correlation between asthmatic response and changes in the mRNA of β-2 receptor in an experimental model of asthma was found.
- 近畿大学の論文
- 1990-06-25
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