<原報>発白血病性化合物 N-ニトロソ-N-ブチル尿素の分解と生体内代謝について : 第 1 報 in vitro における分解について

概要

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The decomposition of N-nitroso-N-butylurea (NBU), recently noted for its high potency to produce leukemia onto mice or rats, was studied in vitro; in the buffer solutions of various pH values from 2 to 10,in horse-blood serum, and in the blood of rat. The decomposition of NBU was found to be the first-order-degradation both in the buffer solutions and in horse-blood serum, and the degradation-constant could be calculated in both cases. It was also shown in the buffer solutions that that the decomposition velocity increased as pH values did and the ratio of increasing of the velocity to that of pH value was maximum in the range of pH value from 6.5 to 7.8. The value of the degradation-constant, 19.5×(10)^<-3> obtained in horse-blood serum was almost identical with that obtained in the buffer solution of pH 6.8,while the recovery of NBU (O-Time) in each case was about 75% and 90% respectively. Some experiments were made in the blood of rat as a preliminary test for in vivo study, in which the recovery of NBU was about 40%. The determination of NBU in the above-described experiments was carried out absorption-spectrophotometrically following with separation using thin layer-or column-chromatography.
共立薬科大学の論文
1970-10-30